Each pipeline below shows what data goes in, what every step does, and what comes back. Share a pipeline page with a colleague or a core facility to show exactly what a run produces.
Quantify gene and isoform expression from paired-end bulk reads with exon-aware alignment and quasi-alignment, ending in count tables and one quality report.
Input
Raw paired-end FASTQ reads
Output
Count tables, Salmon quant files, BAMs, and a MultiQC report
From 10x Genomics reads to an annotated, analysis-ready Seurat object: alignment, QC gating, integration, clustering, automated cell-type labels, and differential expression in one run.
Input
Raw paired-end 10x Genomics FASTQ reads
Output
Annotated Seurat object, H5AD, DGE tables, and reports
Quality checks and resource recommendations for any assay: FastQC on every sample, plus chemistry detection, preliminary cell-quality metrics, and strandedness calls for single-cell and bulk RNA-seq.
Input
Raw FASTQ reads
Output
FastQC reports, resource recommendations, and a MultiQC report
Turn a differential expression result into biological meaning: GO, KEGG and Reactome enrichment, GSEA on the ranked gene list, enrichment plots, and one HTML report per comparison.
Input
A DESeq2-based DGE table per comparison
Output
GO, KEGG and Reactome tables, GSEA results, plots, and an HTML report