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    1. Bridge Horizon
    2. Pipelines

    Pipelines you can run in Horizon

    Each pipeline below shows what data goes in, what every step does, and what comes back. Share a pipeline page with a colleague or a core facility to show exactly what a run produces.

    5 pipelines available in Horizon today

    Available pipelines

    All pipelinesGene expressionSingle-cellImmune repertoireQuality control
    In HorizonImmune repertoire5 steps

    Bulk TCR Detection

    Reconstruct T-cell receptors from paired-end bulk reads with TRUST4, then review clonotypes, productive receptors, and quality reports in one place.

    Input

    Raw paired-end FASTQ reads

    Output

    TCR tables, a repertoire summary, and a MultiQC report

    Explore this pipeline
    In HorizonGene expression8 steps

    Bulk RNA-seq

    Quantify gene and isoform expression from paired-end bulk reads with exon-aware alignment and quasi-alignment, ending in count tables and one quality report.

    Input

    Raw paired-end FASTQ reads

    Output

    Count tables, Salmon quant files, BAMs, and a MultiQC report

    Explore this pipeline
    In HorizonSingle-cell15 steps

    Single-cell RNA-seq

    From 10x Genomics reads to an annotated, analysis-ready Seurat object: alignment, QC gating, integration, clustering, automated cell-type labels, and differential expression in one run.

    Input

    Raw paired-end 10x Genomics FASTQ reads

    Output

    Annotated Seurat object, H5AD, DGE tables, and reports

    Explore this pipeline
    In HorizonQuality control7 steps

    Quality Control

    Quality checks and resource recommendations for any assay: FastQC on every sample, plus chemistry detection, preliminary cell-quality metrics, and strandedness calls for single-cell and bulk RNA-seq.

    Input

    Raw FASTQ reads

    Output

    FastQC reports, resource recommendations, and a MultiQC report

    Explore this pipeline
    In HorizonGene expression6 steps

    Pathway Analysis

    Turn a differential expression result into biological meaning: GO, KEGG and Reactome enrichment, GSEA on the ranked gene list, enrichment plots, and one HTML report per comparison.

    Input

    A DESeq2-based DGE table per comparison

    Output

    GO, KEGG and Reactome tables, GSEA results, plots, and an HTML report

    Explore this pipeline

    Guides and related workflows

    Workflows we explain in detail

    Short guides to the workflows we support, useful when deciding what you want from a run.

    • RNA-seq AnalysisAlignment, quantification, and differential expression on bulk RNA-seq data.
    • Single-cell RNA-seq (Seurat)Cluster, integrate, and annotate single-cell data with Seurat.
    • DESeq2 Differential ExpressionInputs, design formula, contrasts, and cutoffs for a DESeq2 run.
    • nf-core RNA-seq PipelineSamplesheet and reference setup, aligner options, and outputs.

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    Run a pipeline in Horizon

    Sign in to work with your data and launch a run.

    Open Horizon

    Need a tailored pipeline?

    Talk with our team about building or adapting a workflow for your research, including deployment in your AWS environment.

    Contact the team

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