Each pipeline below shows what data goes in, what every step does, and what comes back. Share a pipeline page with a colleague or a core facility to show exactly what a run produces.
Quantify gene and isoform expression from paired-end bulk reads with exon-aware alignment and quasi-alignment, ending in count tables and one quality report.
Input
Raw paired-end FASTQ reads
Output
Count tables, Salmon quant files, BAMs, and a MultiQC report
Turn a differential expression result into biological meaning: GO, KEGG and Reactome enrichment, GSEA on the ranked gene list, enrichment plots, and one HTML report per comparison.
Input
A DESeq2-based DGE table per comparison
Output
GO, KEGG and Reactome tables, GSEA results, plots, and an HTML report